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. 2023 Jul 27;26(9):107490. doi: 10.1016/j.isci.2023.107490

Figure 5.

Figure 5

ACS is required non cell-autonomously for the recovery of the intestinal epithelium after SmDb11 infection

Clonal analyses performed using escargot Flp Out (esgF/O) system.

(A) w;esgGal4,tubGal80ts UAS-GFP;UAS-flp Act>CD2>Gal4 were crossed to UAS-acs-RNAi flies to generate clones of enterocytes expressing both GFP and RNAi against acs (F/O acs). A cell-autonomous mechanism implies that RNAi and wild type enterocytes recover at different rates, presenting a difference between their size 16h post-infection.

(B) Confocal pictures of dissected midguts from F/O control or acs F/O flies at 3h and 16h post-infection and at 16h post sucrose exposure. Clones had been generated for 72h. Blue:DNA; Red:Actin; Green:acs RNAi enterocytes GFP positive. Scale bar = 50μm. Quantification of GFP-positive and negative clones for each condition is represented in (E).

(C–E) Clones had been generated for 24h, 48h, and 72h, respectively; the middle bar of box plots represents the median and the upper and lower limits of the boxes indicate, respectively, the first and third quartile; the whiskers define the minima and maxima. (lmer, ∗∗∗ = p < 0.001, n = 4–7 midguts, merge of two independent replicates; NS: not significant).