Figure 6.

InR and the transcription factor Myc is required for the recovery of the gut enterocytes after infection

NP > ctrl and NP > acs RNAi were exposed to sucrose or infected with SmDb11 for 3h, 8h and 16h. RNA-seq was performed on dissected midguts.

(A) Principal component analyses for gene expression of different samples.

(B) Venn diagram for each time-point comparing genes differentially expressed (fold-change≥2) in NP > ctrl and NP > acs RNAi after infection, relative to sucrose. The scattering of the NP > ctrl data points (A) may limit the power of the analysis and hence lowering the number of significantly differentially regulated genes displayed in the pink circles.

(C) Genes involved in growth that are up- or down-regulated in acs RNAi 16h after infection (relative to sucrose). All genes shown on the graph are differentially expressed with a log2-fold change≥2 and an adjusted p value≤0.05 (see also Table S6).

(D and E) (D) InR and (E) Myc were knocked down in the gut enterocytes (NPiso> InR RNAi and NPiso> Myc RNAi) and compared with the respective controls for the recovery of the gut enterocytes thickness 16h upon infection or exposure to sucrose.

(F) Midguts were dissected from F/O control or Myc F/O flies at 3h and 16h post-infection and at 16h post sucrose exposure. Thickness of GFP-positive and negative clones for each condition was quantified. Clones had been generated for 72h. D-F: the middle bar of box plots represents the median and the upper and lower limits of the boxes indicate, respectively, the first and third quartile; the whiskers define the minima and maxima (lmer, ∗∗∗ = p < 0.001, ∗∗ = p < 0.01, n = 10–22 midguts).