REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Fluorescein Isothiocyanate (FITC)	Sigma-Aldrich	#P5282
Texas-Red labeled phalloidin	Invitrogen TM	#T7471
Mouse anti-GFP antibody	Roche	#11814460001; RRID:AB_390913
Goat anti-mouse FITC antibody	Abcam	#6785; RRID:AB_955241
Rabbit anti-phospho-4EBP1 antibody	Cell Signaling	#2855; RRID:AB_560835
Goat anti-rabbit FITC antibody	Abcam	#6717; RRID:AB_955238
Vectashield	Vector Laboratories	H-1000
Vectashield with DAPI	Vector Laboratories	H-1200
Alexa Fluor 488 alkyne dye	Thermofisher	#A10267
Bacterial and virus strains
Serratia marcescens Db11 (SmDb11)	Flyg et al.52	N/A
Serratia marcescens 21C4 (Sm21C4)	Kurz et al.11	N/A
Biological samples
Intestines	This study	N/A
Chemicals, peptides, and recombinant proteins
Essential amino acids	Thermo Fischer Scientific	MEM Amino Acids Solution 50X #11130051
Non-essential amino acids	Thermo Fischer Scientific	MEM Non-Essential Amino Acids Solution 100X, #11140076
Chloramphenicol	Euromedex	3886-B
Streptomycin	Sigma-Aldrich	S6501
TRI Reagent RT	Molecular Research Center	TR118
Bromoanisole	Molecular Research Center	BAN, 98%,
Critical commercial assays
iScript cDNA synthesis kit	Bio-Rad	#1708890
iQ TM SYBR Green Supermix kit	Bio-Rad	#1525121
L-amino acid quantitation kit	Sigma-Aldrich	#MAK002
Click-it AHA (L-azidohomoalanine) kit	Thermofisher	#C10102
Click-it Cell Reaction Buffer kit	Thermofisher	#C10269
FLIC system (FLIC-MCU+DFM)	Sable Systems Europe	N/A
Deposited data
RNA sequencing WT 3h post-infection	Lee et al.14	GEO Bioproject: GSE60504
RNA sequencing WT 6h and 9h post-infection	This study	GEO Bioproject: PRJNA258425
RNA sequencing WT vs. acs-RNAi at 3h, 8h and 16h post-infection	This study	GEO Bioproject: PRJNA821079
Experimental models: Organisms/strains
Drosophila melanogaster wA5001	Thibault et al.53	N/A
D. melanogaster w1118iso	Laboratory of Luis Teixeira (Ryder et al.)54	N/A
D. melanogaster NP-Gal4-tubGal80ts(also known as Myo31D)	Cronin et al., 2009; Nehme et al., 200712,13	N/A
D. melanogaster NP-Gal4-tubGal80tsiso	This laboratory	N/A
D. melanogaster Ubi-Gal4tubGal80ts	This laboratory	N/A
D. melanogaster Yolk-Gal4	This laboratory	N/A
D. melanogaster prospero-Gal4	Bloomington stock center	#84276
D. melanogaster esg-Gal4Gal80ts	Micchelli&Perrimon55	N/A
D. melanogaster how>Gal4	Bloomington stock center	#1767
D. melanogaster mex1>Gal4	Bloomington stock center	#91368
D. melanogaster uro-Gal4	Laboratory of Bruno Lemaitre (Li et al.)56	N/A
D. melanogaster w; esgGal4tubGal80tsUAS-GFP; UAS-flp Act>CD2>Gal4	Jiang et al.20	N/A
D. melanogaster acs iso CRISPR KO	Sino-French Hoffmann Institute and this laboratory	N/A
D. melanogaster acs iso CRISPR KI	Well Genetics and this laboratory	N/A
D. melanogaster arcus-RNAi (CG1139 RNAi)	VDRC	#102363
D. melanogaster arcus-RNAi iso	This laboratory	N/A
D. melanogaster UAS-CG1139-GFP	Gift from Prof. Deborah Goberdhan17	N/A
D. melanogaster InR RNAi	Bloomington Drosophila Stock Center	#51518
D. melanogaster Myc RNAi	Bloomington Drosophila Stock Center	#51454
D. melanogaster S6K RNAi	Bloomington Drosophila Stock Center	#42572
D. melanogaster S6K-KQ	Bloomington Drosophila Stock Center	#6911
D. melanogaster TOR RNAi	Bloomington Drosophila Stock Center	#33627
D. melanogaster Tsc2 RNAi	Bloomington Drosophila Stock Center	#31776
D. melanogaster Atg9 RNAi	Bloomington Drosophila Stock Center	#28055
D. melanogaster E2f1 RNAi	VDRC	#108837
D. melanogaster E2f2 RNAi	VDRC	#100990
D. melanogaster Egfr RNAi	VDRC	#107130
Oligonucleotides
rp49 fw 5′GACGCTTCAAGGGACAGTATCTG-3′	This laboratory	N/A
rp49 rv 5′- AAACGCGGTTCTGCATGAG3′	This laboratory	N/A
acs fw 5′ACGTCAGCTTTTCGCAGGCCA-3′	This publication	N/A
acs rv 5′-ACAACGCAGCCAGGGTGGAC-3′	This publication	N/A
myc fw 5′ CAGTTCCAGTTCGCAGTCAA-3′	This publication	N/A
myc rv 5′ AGATAAACGCTGCTGGAGGA-3′	This publication	N/A
Software and algorithms
ImageJ/Fiji software	Fiji software	https://imagej.nih.gov/ij/
GraphPad Prism v8.2.1	GraphPad software	https://www.graphpad.com/
CFX384 system	Bio-Rad	1855484
HISAT2	Kim et al.57	https://github.com/DaehwanKimLab/hisat2
Bowtie2	Langmead and Salzberg58	https://bowtie-bio.sourceforge.net/bowtie2/index.shtml
RSEM	Li et al.59	https://github.com/deweylab/RSEM
DEseq2 algorithm	Love et al.60	https://bioinformaticshome.com/tools/rna-seq/descriptions/DESeq2.html#gsc.tab=0
GSEA v4.1.0	Subramanian et al.21	https://www.gsea-msigdb.org/gsea/index.jsp
Other
absorbent pads	Millipore	# AP1003700
DNBseq platform	BGI	N/A