Figure 3.

Multi-tissue and multi-omics approach at the single cell level to better deep phenotype SCAs. From SCA individuals, representative tissue biopsies should be obtained from relevant tissues. Biopsies can then either be dissociated to a single-cell suspension, barcoded and sequenced to obtain single cell omics (A) or sectioned, stained, imaged and barcoded prior to sequencing for spatial RNA expression (B). If possible, clinical measurements should be obtained at the same time for the same individuals (C). Multi-omics and clinical measurements can be combined into an integrated dataset, maintaining both the single cell resolution and the spatial information, thus, making it possible to identify specific molecular signatures for each SCA in a tissue and cell-specific phenotypic context (D). This figure was created with BioRender.com.