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ITC profile generated from the interaction between HRAS1 and HRAS2 IM DNA with fisetin. Upper panel shows the isothermal plot of fisetin and HRAS1 (a) and HRAS2 (b) IM DNA complex formation, and the lower panel represents the integrated binding isotherm generated from integration of the peak area as a function of the molar ratio. The solid line (red) represents the best fit data using the ‘one site binding model’.
