Table 2. Fluorescence Decay Parameters of Fisetin and Morin (10 μM) in Buffer and in the Presence of HRAS1 and HRAS2 IM DNA (20 μM)a.
sample | anisotropy (r) | IHH | IHV | IVH | IVM | IVV | G factor |
---|---|---|---|---|---|---|---|
10 μM fisetin | 0.204 | 8484 | 9429 | 7187 | 8637 | 13964 | 1.11 |
10 μM fisetin + 20 μM HRAS1 | 0.280 | 8421 | 9584 | 7081 | 9378 | 16904 | 1.13 |
10 μM fisetin + 20 μM HRAS2 | 0.225 | 11651 | 13382 | 9792 | 12339 | 20526 | 1.14 |
10 μM morin | 0.132 | 4941 | 2654 | 3854 | 3832 | 2998 | 0.53 |
10 μM morin + 20 μM HRAS2 | 0.154 | 5296 | 2844 | 4180 | 4169 | 3507 | 0.53 |
10 μM morin + 20 μM HRAS1 | 0.137 | 5337 | 2892 | 4138 | 4118 | 3295 | 0.54 |
IVV, IVH, IHV, and IHH represent fluorescence signals for excitation and emission with the polarizer set at positions (0,0°), (0,90°), (90,0°), and (90,90°), respectively, and G is the sensitivity factor of the detection systems.