Table 3. Fluorescence Decay Parameters of Fisetin and Morin (10 μM) in Buffer and in the Presence of Different Concentrations (10 and 20 μM) of HRAS1 and HRAS2 IM DNA.
sample | τ1 (ns) | α1 | τ2 (ns) | α2 | τ3 (ns) | α3 | τavg (ns) | χ2 |
---|---|---|---|---|---|---|---|---|
10 μM fisetin in buffer | 0.03 | 0.45 | 0.36 | 0.55 | --- | --- | 0.19 | 1.05 |
10 μM fisetin+ 10 μM HRAS1 | 1.25 | 0.40 | 5.56 | 0.49 | 0.15 | 0.11 | 2.32 | 0.98 |
10 μM fisetin+ 20 μM HRAS1 | 1.52 | 0.37 | 5.84 | 0.48 | 0.27 | 0.15 | 2.54 | 0.94 |
10 μM fisetin+ 10 μM HRAS2 | 1.12 | 0.51 | 0.09 | 0.20 | 4.25 | 0.29 | 1.82 | 0.99 |
10 μM fisetin+ 20 μM HRAS2 | 1.13 | 0.51 | 1.00 | 0.19 | 4.24 | 0.30 | 2.12 | 0.95 |
10 μM morin in buffer | 0.6 | 0.33 | 0.2 | 0.57 | 5.27 | 0.10 | 2.02 | 0.93 |
10 μM morin+ 10 μM HRAS2 | 0.8 | 0.26 | 5.55 | 0.15 | 0.25 | 0.59 | 2.20 | 0.91 |
10 μM morin+ 20 μM HRAS2 | 0.9 | 0.24 | 5.79 | 0.18 | 0.27 | 0.58 | 2.32 | 0.93 |
10 μM morin+ 10 μM HRAS1 | 0.8 | 0.28 | 4.92 | 0.12 | 0.24 | 0.60 | 1.98 | 0.92 |
10 μM morin+ 20 μM HRAS1 | 1.00 | 0.27 | 4.82 | 0.15 | 0.24 | 0.58 | 2.02 | 0.91 |