Figure 9.

MTOR inhibition does not induce SMAD2 linker phosphorylation in SC cells. Western blot analysis showing protein expression levels of pSMAD2L in human SC cells treated and rapamycin. SC cells were treated with an MTOR inhibitor (rapamycin; 1 μM) and subjected to shear stress (10 dyn/cm2) for 1 h. Protein levels of pSMAD2L and pp70S6K were measured by western blot analysis. ACTB was used for normalization. Data are shown as the mean ± S.D (n=3). *, p<0.05; ns, not significant (Student’s t-test).