Fig. 2.

Key features and functional requirements of OoC microfluidic devices for 2D cultures relevant to barrier tissues e.g. epithelium and endothelium, and 3D cultures relevant to parenchyma and connective tissues e.g. liver, bone, heart. Connections between the fluidic networks to external pumps, valves and other devices are typically facilitated via commercial fitting (e.g. Luer fittings) at the cost of device footprint due to their larger liquid volume. Customized slip-fit connectors can be utilized to reduce device footprint.