Extended Data Fig. 8 |. Task representations show increased stability with learning following a break in training.

a. In the 2-odor task, Late and Reacquisition sessions were separated by multiple extinction sessions. To assess how task representations may change across a similar time period, but with no additional task experience, following learning of the 4-odor task, mice were kept in their homecage and rerun on the learned task 4 days later (Post). b. Mean lick rate during the trace period for all animals (n = 8 vCA1, 5 dCA1 mice,, two sided Mann-Whitney U test, * p < 0.05, *** p < 0.001, error bars mean ± SEM). c, d. Trial-type and CS+ vs CS- decoding accuracies were similar for the Post session (shown here) compared to Late (Fig. 5c and Extended Data Fig. 7a, b; Analyses used 150 cells for each region). e As in Late session, odor and outcome information were multiplexed in vCA1 during the odor delivery period, while outcome information was present in both vCA1 and dCA1 during trace (n = 10 decoding iterations from n-matched 150 cells from 8 vCA1 and 5 dCA1 mice, two sided Mann-Whitney U test, *** p < 0.001, error bars are mean ± SEM). f. Pearson’s correlation of activity patterns across time bins. g. Task representations showed greater stability once learned. Analyses used cells registered across all 3 sessions. (n = 10 decoding iterations from n-matched 100 cells from 8 vCA1 and 5 dCA1 mice, two sided Mann-Whitney U test, * p < 0.05, ** p < 0.01, *** p < 0.001, error bars are mean ± SEM). h. Same as in g, but decoding CS+ vs CS- across sessions. See Supplementary Table 1 for all statistical analysis details.