FIG. 5.

Protein synthesis in virus-infected HeLa cells. Cells infected with the wild-type, SPC45, and ICR1629 viruses (MOI of 10) were grown at either 34 or 40°C and labeled with [³⁵S]methionine (10 μCi/ml) for 30 min beginning from 3, 5, and 6 h p.i., as shown above the panels. For other experimental details, see reference 10. Lanes m1 and m2, cells mock infected and labeled during 6 to 6.5 h p.i. at 34 and 40°C, respectively. Ps, virus polymerase proteins. HA, hemagglutinin.