FIG 9.

Other STARR-seq-identified motifs contribute only to basal GADD45A enhancer activity. (A) Schematic representation of the GADD45A-nLuc reporter construct with relevant enhancer sequence motifs highlighted in panel B. (C) Normalized luciferase expression values using GADD45A-nLuc reporter transfected into HCT116 WT cell line and 16 h treatment with DMSO, nutlin-3A and tunicamycin as indicated in the legend. Reporter constructs included wild-type (WT) construct, 250 bp enhancer deletion (“No Enhancer”) as a negative control and various predicted transcription factor motif mutations as indicated on the x-axis. Specific mutations in transcription factor motifs based on the STARR-seq screen are indicated in panel B. Statistical comparisons were generated using one-way ANOVA: *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.