Table 3. Bioassay of disinfected 263K coated wires in tg7 mice.

Disinfectant	Exposure Time (min)	Dilution of 263K brain homogenate used to coat wires, prior to treatmenta					Titerb	Log10 Reduction in titer
		10−1	10−4	10−5	10−6	10−7
None	NA	5/5 c, 65	4/4, 83	4/4, 95.5	5/5, 142	1/8, 125	6.6	NA
4% Wex-cide	2	0/4	nt	nt	nt	nt	< 0.5	≥6.1
4% Wex-cide	30	0/4	nt	nt	nt	nt	< 0.5	≥6.1
2% eLpH	2	0/7	nt	nt	nt	nt	< 0.5	≥6.1
2% eLpH	30	0/6	nt	nt	nt	nt	< 0.5	≥6.1

^a Steel wires were exposed to 263K prion infected brain homogenates, then washed, dried, and immersed in different disinfectants for either 2 or 30 minutes. Following treatment wires were removed and allowed to dry. Each mouse was implanted intracerebrally with a single 3–4 mm wire.

^b The calculated titer reported is the log₁₀LD₅₀ / wire

^c The numerator is the number of prion-positive mice (see Methods), and the denominator is the number of mice implanted. For groups with positive mice the average incubation period in days-post inoculation (dpi) is provided. Tg7 mice typically do not develop 263K prion disease after 200 dpi. Mice in this experiment that did not develop clinical signs of prion disease were euthanized at 315 dpi.

NA: not applicable, nt: not tested