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. 2023 Aug 24;18(8):e0290325. doi: 10.1371/journal.pone.0290325

Table 5. Bioassay of disinfected 22L brain homogenate in tga20 mice.

Disinfectant Dilution of 22L brain homogenate inoculated after treatmenta Titerb Log10 Reduction in titer
10−3 10−4 10−5 10−6 10−7 10−8 10−9
Saline 4/4 c, 94 4/4, 104 4/4, 112 4/4, 126 4/4, 156 1/4, 149 0/4 9.25 NA
4% Wex-cide 1/8, 228 0/8 nt nt nt nt nt 4.15 5.1
2% eLpH 8/8, 158 2/7, 174 nt nt nt nt nt 5.3 4.0

aAliquots of 22L-infected brain homogenates (10%) were exposed to different disinfectants or saline for 30 minutes at a 1:9 ratio. Solutions were then further diluted for bioassay in mice. Each recipient mouse received 30μl of inoculum.

b The calculated titer reported is the log10LD50 / gram of tissue

c The numerator is the number of prion-positive mice (see Methods), and the denominator is the number of mice inoculated. For groups with positive mice the average incubation period in days-post inoculation (dpi) is provided. Tga20 mice typically do not develop 22L prion disease after 200 dpi. Mice that did not develop clinical signs of prion disease were euthanized at 289 dpi.

NA = not applicable, nt = not tested