Table 6. Bioassay of disinfected sCJD brain homogenate in tg66 mice.

Disinfectant	Dilution of sCJD brain homogenate inoculated after treatmenta					Titerb	Log10 Reduction in titer
	10−3	10−4	10−5	10−6	10−7
Saline	4/4 c, 204	4/4, 296	3/4, 416	0/4	0/4	6.77	NA
40% Wex-cide	nt	1/5, 434	0/4	0/4	nt	5.22	1.5
4% Wex-cide	4/4, 309	2/4, 372	0/4	0/5	nt	5.52	1.25
2% eLpH	3/4, 453	3/4, 481	0/4	nt	nt	5.52	1.25

^aAliquots of sCJD prion infected brain homogenates (10%) were exposed to different disinfectants or saline for 30 minutes at a 1:9 ratio. Solutions were then further diluted for bioassay in mice. Each recipient mouse received 30 μl of inoculum.

^b The calculated titer reported is the log₁₀LD₅₀ / gram of tissue

^c The numerator is the number of prion-positive mice (see Methods), and the denominator is the number of mice inoculated. For groups with positive mice the average incubation period in days-post inoculation (dpi) is provided. Tg66 mice typically do not develop sCJD after 550 dpi. Mice that did not develop clinical signs of prion disease were euthanized at 650 dpi.

NA = not applicable, nt = not tested