Table 7. Bioassay of disinfected sCJD coated wires in tg66 mice.

Disinfectant	Exposure Time (min)	Dilution of sCJD brain homogenate used to coat wires, prior to treatmenta
		10−1	10−3	10−4	10−5
None	NA	4/4 c, 319	2/3, 311	4/4, 348	4/4, 360
4% Wex-cide	2	1/6, 496	nt	nt	nt
4% Wex-cide	30	0/6	nt	nt	nt
2% eLpH	2	5/6, 391	nt	nt	nt
2% eLpH	30	0/6	nt	nt	nt
Water	30	6/6, 303	nt	nt	nt

^a Steel wires were exposed to sCJD prion infected brain homogenates, then washed, dried, and immersed in different disinfectants for either 2 or 30 minutes. Following treatment wires were removed and allowed to dry. Each mouse was implanted intracerebrally with a single 3–4 mm wire.

^b The calculated titer reported is the log₁₀LD₅₀ / wire

^c The numerator is the number of prion-positive mice (see Methods), and the denominator is the number of mice inoculated. For groups with positive mice the average incubation period in days-post inoculation (dpi) is provided. Tg66 mice typically do not develop sCJD after 550 dpi. Mice that did not develop clinical signs of prion disease were euthanized at 650 dpi.

NA = not applicable, nt = not tested