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. 2023 Aug 24;18(8):e0290675. doi: 10.1371/journal.pone.0290675

Table 1. Primers used for generation of SARS-CoV-2 RNA segments.

RNA Forward Primer Reverse primer
RNA 1 CATGAGCTTA AGTAATACGA CTCACTATAG GCTACTAACA ATGCCAT CATGAGGCTA GCAAGATTGT GTCCGCTTAA AA
RNA 2 CATGAGCTTA AGTAATACGA CTCACTATAG ACCTGAGCAT AGTCTTG CATGAGGCTA GCGAGAACAC ACAGCCTC
RNA 3 CATGAGCTTA AGTAATACGA CTCACTATAG TGAAGTGCTG TCTGAC CATGAGGCTA GCGTTACACG ATAACCAGTA AAG

Primers used to amplify RNAs 1–3 prior to subcloning as described above.