Table 1. Primers used for generation of SARS-CoV-2 RNA segments.
RNA | Forward Primer | Reverse primer |
---|---|---|
RNA 1 | CATGAGCTTA AGTAATACGA CTCACTATAG GCTACTAACA ATGCCAT | CATGAGGCTA GCAAGATTGT GTCCGCTTAA AA |
RNA 2 | CATGAGCTTA AGTAATACGA CTCACTATAG ACCTGAGCAT AGTCTTG | CATGAGGCTA GCGAGAACAC ACAGCCTC |
RNA 3 | CATGAGCTTA AGTAATACGA CTCACTATAG TGAAGTGCTG TCTGAC | CATGAGGCTA GCGTTACACG ATAACCAGTA AAG |
Primers used to amplify RNAs 1–3 prior to subcloning as described above.