TABLE 1.
Dose-mortality responses of neonate S. frugiperda and T. ni larvae infected orally with OBs of wt AcMNPV or ie-2 mutant virusesa
Host and virus | LC50b (OBs/ml) | 95% Fiducial limit
|
Slope (mean ± SE) | Heterogeneity factorc | |
---|---|---|---|---|---|
Upper | Lower | ||||
S. frugiperda | |||||
wt AcMNPV | 2.6 × 105 | 4.2 × 105 | 1.5 × 105 | 1.148 ± 0.177 | 0.31 |
vie2d(94-274) | 3.9 × 108 | 9.9 × 108 | 2.5 × 108 | 1.131 ± 0.224 | 0.34 |
vie2d(94-173) | 3.1 × 108 | 5.2 × 108 | 2.0 × 108 | 1.142 ± 0.193 | 0.30 |
vie2d(215-274) | 1.2 × 109 | 1.9 × 109 | 0.5 × 109 | 0.756 ± 0.210 | 0.12 |
T. ni | |||||
wt AcMNPV | 1.4 × 104 | 1.8 × 104 | 1.0 × 104 | 2.941 ± 0.514 | 0.40 |
vie2d(94-274) | 1.3 × 106 | 1.9 × 106 | 0.9 × 106 | 1.554 ± 0.225 | 0.42 |
vie2d(94-173) | 1.8 × 106 | 2.8 × 106 | 0.8 × 106 | 1.715 ± 0.386 | 0.79 |
vie2d(215-274) | 3.1 × 106 | 4.2 × 106 | 2.3 × 106 | 1.926 ± 0.249 | 0.16 |
Six groups of neonates (less than 12 h old) were allowed to feed on diet contaminated with various concentrations of OBs per ml for 24 h and then transferred to uncontaminated diet. The number of dead larvae at 10 days p.i. was used to determine the LC50.
Values were calculated by probit analysis.
χ2 divided by the degrees of freedom (POLO-PC; LeOra Software).