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. Author manuscript; available in PMC: 2023 Aug 24.
Published in final edited form as: J Allergy Clin Immunol. 2022 Dec 29;151(4):1040–1049.e5. doi: 10.1016/j.jaci.2022.11.027

FIG 3.

FIG 3.

Recombinant expression of C1-INH variants. A, WB analysis of supernatants from transfected WT C1-INH and variant constructs in reducing conditions. Recombinant expressions of P399A, P399A/L349F, and F471C are markedly decreased (~90% compared with that of WT). The expressions of C130W and N272del are decreased by approximately 50% compared with that of WT. K273del and L349F have normal secretion compared with that of WT (Table II). B, WB analysis of supernatants from transfected WT and variant constructs in nonreducing conditions. I224S is secreted and forms oligomers. This experiment was repeated 4 times with similar results.