FIG. 2.

Increase in intracellular Ca²⁺ concentration and plasma membrane permeability to Ca²⁺ in rotavirus-infected MA104 and HT29 cells. At 7 h postinfection, MA104 (A) and HT29 (B) monolayers were trypsinized, and cell suspensions were loaded with fura-2 for the measurement of [Ca²⁺]_i (see Materials and Methods). Permeability to Ca²⁺ in rotavirus (V)- or mock (M)-infected cells was evaluated by the change in [Ca²⁺]_i induced by the addition of 5 mM CaCl₂ to the extracellular medium (arrow), which initially contained 1 mM Ca²⁺. Results of a representative experiment of a series of three for each cell type are shown.