TABLE 1.

Changes of intracellular Ca²⁺ concentration following an extracellular Ca²⁺ increase in mock- and rotavirus-infected MA104 cells^a

Infection	[Ca2+]out addition (mM)	[Ca2+]i change		Time to d[Ca2+]i/ dtmax (s)	n
		Δ amplitude at 20 s (nM)	d[Ca2+]i/dtmax (nM/s)
Mock	2	35 ± 2	7.2 ± 1.0	8.6 ± 0.4	3
	5	48 ± 4	8.2 ± 0.5	9.7 ± 0.4	8
	30	63 ± 6	10.5 ± 1.1	9.8 ± 0.6	5
Virus	2	98 ± 5*	23.6 ± 1.4*	7.5 ± 0.4†	3
	5	188 ± 21*	39.7 ± 4.2*	9.8 ± 0.9†	8
	30	214 ± 47*	50.2 ± 10.8*	9.2 ± 0.4†	5

^aIntracellular Ca²⁺ concentration and permeability in mock- and rotavirus-infected MA104 cells were determined at 7 h postinfection as detailed in the legend to Fig. 3, with extracellular Ca²⁺ concentration changes of 2, 5, and 30 mM. The change in [Ca²⁺]_i was determined in each individual experiment at 20 s after the change in extracellular Ca²⁺ concentration (Δ amplitude). The peak value of the first derivative of the original traces corresponds to the maximal rate of [Ca²⁺]_i increase after Ca²⁺ addition (d[Ca²⁺]_i/dt_max). The time from Ca²⁺ addition to attain this last value was computer determined in each experiment from derivative curves like the ones shown in Fig. 3. Values are means ± SEMs from n experiments in each case. *, significantly different from the corresponding value for mock-infected cells by the Mann-Whitney test (P < 0.05); †, not significantly different from the corresponding value for mock-infected cells.