TABLE 3.
Uptake of 45Ca2+ and 51Cr3+ in mock- and rotavirus-infected MA104 cellsa
Infection | Cation | cpm at:
|
||
---|---|---|---|---|
4 hpi | 6 hpi | 8 hpi | ||
Mock | 45Ca2+ | 223 ± 9.7 | 174 ± 13.9 | 234 ± 21.0 |
51Cr3+ | 118 ± 4.3 | 89.3 ± 4.6 | 102 ± 4.6 | |
Virus | 45Ca2+ | 225 ± 15.4† | 256 ± 11.5* | 532 ± 21.0* |
51Cr3+ | 104 ± 1.9† | 105 ± 4.8† | 117 ± 8.3† | |
Ratio (virus/mock) | 45Ca2+ | 1.0 | 1.5 | 2.3 |
51Cr3+ | 0.9 | 1.2 | 1.1 |
MA104 cell monolayers were grown to confluency in 24-well Linbro plates and infected. At 4, 6, or 8 h postinfection (hpi), 45Ca2+ or 51Cr3+ uptake was determined (see Materials and Methods for details). The time of uptake was 10 min in each case. Values correspond to the means ± SEMs from four independent measurements in each case. The virus/mock ratio was calculated from the mean values for each ion at the different times postinfection. *, significantly different from the corresponding value for mock-infected cells by the Mann-Whitney test (P < 0.05); †, not significantly different from the corresponding value for mock-infected cells.