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. 2023 May 23;8(4):e00152-23. doi: 10.1128/msphere.00152-23

Fig 5.

Fig 5

MRST disruption leads to the dysregulation of ribosome biogenesis pathways, causing downstream effects on translation expression. (a) Due to the bioinformatically predicted homology of the MRST protein to the midasin protein linker domain and disordered regions, ribosome metabolism pathway expression was analyzed at 6 hpi. While only 13 out of 97 genes (~13%) related to ribosome structure were downregulated at 6 hpi, 51 out of 133 genes (~38%) of the ribosome assembly pathway were downregulated, primarily pointing to the dysregulation of ribosomal assembly/maturation, and not the ribosome subunits themselves, being affected by MRST disruption. (b) Several tRNA ligases were differentially expressed at 6 hpi, with the majority lacking significant dysregulation at the other harvested timepoints in the mutant (P-value > 0.05). (c) Seven genes associated with RNA polymerase III activity (PF3D7_0609900, PF3D7_0708100, PF3D7_1104700, PF3D7_1206600, PF3D7_1210400, PF3D7_1329000, PF3D7_1449300) were dysregulated at 6 hpi compared to the other timepoints. Graphs and statistics are generated in GraphPad Prism.