Fig 3.

Activity of PlyB against other Bacillus spp. and Gram-positive ocular pathogens. Four Bacillus spp. (B. cereus ATCC 14579, a B. cereus ocular isolate, B. thuringiensis, B. subtilis, and B. megaterium) and three Gram-positive (Gm+) ocular isolates (S. aureus, E. faecalis, and S. pneumoniae) were tested against PlyB. Bacteria were grown overnight, washed, and incubated with PlyB at 125 µg/mL in PBS, pH 7.4 for 1 hour at 37°C. Bacteria were quantified by serial dilution and plating. PlyB completely killed only B. cereus and B. thuringiensis but not the other Bacillus strains or Gram-positive organisms. Values represent means ± SEM of n ≥5 with at least two independent experiments. *P ≤ 0.05, ns >0.05.