Fig 4.

Activity of Bacillus phage lysin PlyB in different microbial growth conditions. B. cereus ATCC 14579 was grown overnight, diluted at 1:100, and grown to midlogarithmic phase. The bacteria were harvested, washed, and resuspended in PBS, BHI, LB, DMEM, Vit, and human plasma-like medium (plasma) to an OD₆₀₀ of ~2.0. Bacteria were diluted 1:1 in a 96-well plate with either buffer only (no PlyB control) or PlyB at a final concentration of 125 µg/mL. The OD₆₀₀ was measured at 10 minutes. PlyB significantly reduced the bacterial turbidity within 10 minutes of incubation. Values represent means ± SEM of n ≥5 with at least two independent experiments.*P ≤ 0.05, ns >0.05.