Fig 6.

PlyB did not activate TLR2 and TLR4. HEK-Blue hTLR2 (A and C) and hTLR4 (B and D) reporter cells were incubated with PlyB, PlyB-incubated B. cereus (PlyB-T), GAT, GAT-incubated B. cereus (GAT-T), and B. cereus envelope (BC env). An oxidized phospholipid OxPAPC (TLR2 and TLR4 inhibitor) was also added to each group to assess inhibition of TLR2 and TLR4 activation. PlyB and GAT did not activate TLR2 and TLR4. TLR2 and TLR4 activation by PlyB-incubated and GAT-incubated B. cereus were significantly less than their respective positive (+ve) controls. OxPAPC significantly reduced TLR2 and TLR4 activation by the PlyB-incubated or GAT-incubated B. cereus. Values represent mean ± SEM of N ≥4 for at least two separate experiments; *P < 0.01 and ns >0.05.