FIGURE 1.

Effects of injury and sex on muscle size. (a) Tibialis anterior muscles of 3‐month‐old male (M) and female (F) mice were injured with BaCl₂ injection and collected 7 and 14 days after injury (AI). (b) Representative H&E images of cross‐sections of injured and uninjured control (CNT) muscles. (c–e) The cross‐sectional area (CSA) of myofibers was measured from the H&E images (c) and presented as a percentage of uninjured control (d) or on a histogram (e) (n = 5–7). Data are presented as mean ± SEM. Statistical significance was determined by two‐way ANOVA followed by Student–Newman–keuls post hoc test (c) or unpaired t‐test (d). *p < 0.05, **p < 0.01, ***p < 0.001 versus males at each time point; ^†† p < 0.01, ^††† p < 0.001 versus CNT.