Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jun 8;19(9):1147–1157. doi: 10.1038/s41589-023-01350-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Extended Data Fig. 7 — a, d. Confocal images of U-2 OS cells expressing ChemoG-NAD in the nucleus (ChemoG-NAD-NLS, a) or mitochondria (ChemoG-NAD-mito, d) labeled with SiR. Shown are the eGFP channel, FRET channel and ratio image (FRET/eGFP) in pseudocolor (LUT = mpl-viridis). Cells were treated for 24 h either with DMSO (Ctrl), 100 nM FK866 or 1 mM NR. All scale bars = 25 μm. b, e. FRET/eGFP ratios of U-2 OS cells corresponding to panels a and d, respectively. Shown are the FRET/eGFP ratios of single cells (circles) and the mean ±s.d. (black line) (nucleus: n = 353 (ctrl), 311 (NR), 300 (FK866) cells; mitochondria: n = 63 (ctrl), 85 (NR), 109 (FK866) cells; from 3 independent experiments). p-values are given based on unpaired two-tailed t-test with Welch’s correction (**** p < 0.0001). c. Confocal images of U-2 OS cells expressing ChemoG-NAD in the nucleus (ChemoG-NAD-NLS) and stained with Hoechst. f. Confocal images of U-2 OS cells expressing ChemoG-NAD in the mitochondria (ChemoG-NAD-mito) and stained with MitoTracker RedFM. All scale bars = 25 μm.

Source data