TABLE 1.

Replication kinetics of wild-type and Δtat HIV-1^a

Day	p24 Ag (pg/ml) with the following virus:
	Wild type	Δtat	tat wild type	[E2G, D5G, E9G]	P3L	P[6, 10]L	P[10, 14]L	C275	K41A	K/R[50-57]G	Mock
0	5	15	20	30	20	15	20	20	20	15	0
3	150	0	0	0	0	0	0	0	0	0	0
7	750	0	0	0	0	0	0	0	0	0	0
10	3,598	0	0	0	0	0	0	0	0	0	0
14	48,763	0	0	0	0	0	0	0	0	0	0
17	24,851	0	0	0	0	0	0	0	0	0	0
21	36,891	0	0	0	0	0	0	0	0	0	0

^aActivated PBMCs were infected with virus stocks containing 60 mU of RT activity. The cells were washed and cultured as described in Materials and Methods, and a sample of culture supernatant was removed and assayed for p24 Ag (day 0) by ELISA. The cells were split 1:2 twice weekly, and an aliquot of the supernatant was assayed for p24 Ag. All infections were supplemented with uninfected, activated PBMCs weekly. Each infection was repeated twice with independent virus stocks.