Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Aug 24;14:5176. doi: 10.1038/s41467-023-40861-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — a Workflow of hFOs culture in this study. Scale bar, 200 μm and 300 μm. b, c RT-qPCR analysis for PCCB expression in hiPSCs (b) and hFOs (c), n = four technical replicates per group. d Immunostaining characterization for representative control hFOs. N ≥ three biologically independent samples in each group. Cortical plate marker, MAP2; intermediate zone marker, TBR2; ventricular zone markers, SOX2 and ki67; forebrain-specific markers, FOXG1 and PAX6. Scale bar, 50 μm. e PCA plot for the PCCB knockdown and control hFOs. f Volcano plot of DEGs between the PCCB knockdown and control hFOs. Upregulated genes are shown with red dots and downregulated genes are shown with blue dots. g, h GO and KEGG analysis for the PCCB knockdown-induced upregulated (g) and downregulated DEGs (h), respectively. GO terms are shown with red bars, and KEGG pathways are shown with blue bars. i PPI network analysis for 350 downregulated DEGs shared in both PCCB-G1 and PCCB-G2 hFOs. The top 10 hub nodes are shown with orange nodes. j RT-qPCR analysis for GABRA1, GABRA2, GABRB2, and GABRB3 (The hub nodes in the PPI network), n = four technical replicates per group. Data are shown as Mean ± SD. The unpaired two-tailed t-test was used to assess difference between the PCCB-NC and PCCB-G1 or PCCB-G2 group. **P < 0.01, ***P < 0.001, ****P < 0.0001. Source data underlying b, c, and j are provided as a Source Data file.