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. 2023 Aug 24;13:13861. doi: 10.1038/s41598-023-37248-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Orthogonal viewing by x-, y-, and z-slicing. (A) An xz-plane image reveals the SC’s cross-sectional area change and branching of CC stemming from the SC, as compared to Fig. 1D. Note shadow effect is evident which is cast by the damaged tissue on the tissue surface. (B) An x-slicing results in a yz-plane image, where the SC is clearly visualized as a low-intensity band right above the TM. Note CC is running approximately parallel above the SC. (C) A z-slicing through the center of SC from the top view allows for visualization of the juxtacanalicular tissue and inner wall of SC, which are considered the main source of resistance of the human ocular outflow. Also straightforward is that the TM locates at a relatively deeper depth than the nearby sclera. TM: trabecular meshwork; SC: Schlemm’s canal; CC: collector channel; AC: anterior chamber. More details such as the cross-sectional area changes of SC, distribution and branching of CCs, the morphology of TM/SC/CC region, and nearby vessels can be seen in video1_radialStack, and video2_3DSlice. Same eye used in Fig. 1.