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. 2023 Aug 24;14:5147. doi: 10.1038/s41467-023-40924-4

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Representative genome browser tracks (left) and quantification (right) of WRNIP1 ChIPseq experiments. WRNIP1 ChIPseq tags were quantified at genic regions of all genes and displayed as scaled metagenes. b WRNIP1 ChIPseq normalized tag counts for the top 1000 WRNIP1-bound genes in HUWE1-WT cells compared with HUWE1-CS cells (n = 1000 genes). Boxplots represent median±quartiles with whiskers ranging from minimum to maximum values. Significance was determined using a paired, two-tailed t test. c Immunoprecipitation (IP) analysis with WRNIP1 in benzonase-treated HCT116 lysates (n = 3). d PLA with antibodies to WRNIP1 and RNAPII (n = 202, 221, 230, 182 cells), pS2-RNAPII (n = 179, 216, 232, 207 cells) and pS5-RNAPII (n = 181, 202, 252, 182 cells) in HUWE1-WT and HUWE1-CS cells expressing shWRNIP1 (shW1) or a shCtrl. Boxplots show median±quartiles with whiskers ranging up to 1.5-fold of the inter-quartile range. P-values were determined using Kruskal-Wallis test followed by Dunn’s multiple comparison. e Re-ChIP experiment consisting of a first ChIP with WRNIP1 antibodies followed by elution of protein-DNA complexes and a second ChIP with RNAPII antibodies. Purified DNA was analyzed by qPCR with the indicated primer pairs (n = 3, mean ± SD). f Immunoprecipitation of total, pS2-, or pS5-RNAPII in formaldehyde-crosslinked WT and CS cells (n = 2). g ChIPseq analysis of pS2-RNAPII in the indicated cell lines. Representative genome browser tracks (left) and quantification of pS2-RNAPII ChIPseq tag coverage at all transcription termination sites (right). Source data are provided as a Source Data file.