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. 2023 Aug 24;14:5147. doi: 10.1038/s41467-023-40924-4

Fig. 4. HUWE1 and WRNIP1 suppress ATM signaling.

Fig. 4

a Immunoblotting analysis of HUWE1-WT and HUWE1-CS cells expressing shWRNIP1 or shCtrl (n = 3). b Neutral comet assay of HUWE1-WT and HUWE1-CS cells with or without WRNIP1 depletion (n = 1395, 1045, 779, 660 comets). Boxplots (right) show median±quartiles with whiskers ranging up to 1.5-fold of the inter-quartile range. P-values were determined using Kruskal–Wallis test followed by Dunn’s multiple comparison. Scale bar: 50 µm. c Representative tracks (left) and quantification of DSBCapture tag density (right) in HUWE1-WT and HUWE1-CS cells expressing shWRNIP1 or shCtrl. d Metagene analysis of WRNIP1 ChIPseq coverage for all genes and genes with DSBCapture peaks in HUWE1-WT cells. e Immunoblotting analysis of WT and CS cells expressing shCtrl or shWRNIP1, treated with 2.5 µM ATM inhibitor KU55933 or DMSO for 2 h (n = 3). f Analysis of proliferation of HUWE1-WT or HUWE1-CS cells expressing shCtrl or shWRNIP1 by crystal violet staining (n = 3, mean ± SD). Source data are provided as a Source Data file.