Table 1.
Antibodies used in this study
| Target | Catalog number | Manufacturer | Reactivity | Application |
|---|---|---|---|---|
| 53BP1 | 4937 | Cell Signaling | H Mk | WB, IHC, IF |
| ATM (G-12) | sc-377293 | Santa Cruz | H | WB, IP, IF, IHC(P) and ELISA |
| ATR | 2790 | Cell Signaling | H Mk | WB |
| BrdU (B44) | BD347580 | BD | Species independent | Flow cytometry, Intracellular staining (flow cytometry) |
| BrdU [BU1/75 (ICR1)] | ab6326 | Abcam | Species independent | ICC/IF, Flow Cyt (Intra), IHC-P |
| CDC45 | 11881S | Cell Signaling | H M R Mk | WB, IP, IF |
| CHK2 | 2662T | Cell Signaling | H M R Mk | WB, IP |
| H2AX (938CT5.1.1) | sc-517336 | Santa Cruz | H | WB, IP, IF, and IHC(P) |
| HA-tag (6E2) | 2367 | Cell Signaling | Species independent | WB, IHC, IF, F |
| HA-tag (C29F4) | 3724 | Cell Signaling | Species independent | WB, IP, IHC, IF, F, ChIP |
| Histone H2B (53H3) | 2934 | Cell Signaling | H M R Mk Z | WB |
| Histone H3 (96C10) | 3638 | Cell Signaling | H M R Mk | WB |
| HUWE1 | A300-486A-A | Bethyl | H | WB, IP, IHC |
| KAP1 | 15202-1-AP | Proteintech | H M R | WB, IP, IHC, IF, FC, ChIP, ELISA |
| MCM2 (D7G11) | 3619S | Cell Signaling | H M R Mk | WB, IP, IHC, IF, ChIP |
| MCM5 | ab17967 | Abcam | H | WB, IHC-P |
| MRE11 | NB100-142 | Novus Biologicals | Hu, Mu, Rt, Ch, Ha, Bt, Bv, Ca, Pm, Eq, Fe, Pm | WB, Simple Western, ChIP, ELISA, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, KD, KO |
| MRE11 (18) | sc-135992 | Santa Cruz | H M R | WB, IP, IF |
| MYC (D3N8F) | 13987 | Cell Signaling | H M R Mk | WB, IF, F, ChIP, C&R |
| PCNA (PC10) | sc-56 | Santa Cruz | H M R Y | WB, IP, IF, IHC(P) and FCM |
| pS139-H2AX (20E3) | 9718 | Cell Signaling | H M R Mk | WB, IHC, IF, F |
| pS139-H2AX | 517348 | Santa Cruz | H M R | WB, IP and IF |
| pS1981-ATM (10H11.E12) | 47739 | Santa Cruz | H M R | WB, IP, IF and IHC(P) |
| pS1981 ATM (EP1890Y) | ab81292 | Abcam | H | Dot blot, Flow Cyt (Intra), WB, IHC-P, IP |
| pS2-RNAPII | 61083 | Active Motif | H M | WB, IP, IF, ChIP, ICC |
| pS2-RNAPII | A300-654A-M | Bethyl | H M | WB, IP, IHC |
| pS25-53BP1 (38.Ser 25) | sc-135748 | Santa Cruz | H M | WB, IP and ELISA |
| pS4/8-RPA2 (E5A2F) | 54762 S | Cell Signaling | H | WB, IF, F |
| pS428-ATR | 2853 T | Cell Signaling | H M R Mk | WB |
| pS5-RNAPII | 39749 | Active Motif | H M | WB, ChIP |
| pS824-KAP1 (BL-246-7B5) | ab243870 | Abcam | H M | IP, IHC-P, WB, ICC/IF |
| pT68-CHK2 | ab3501 | Abcam | H | WB |
| RAD50 (G-2) | sc-74460 | Santa Cruz | H M R | WB, IP, IF and ELISA |
| RAD50 | NB100-154 | Novus Biologicals | Hu, Mu, Ha | WB, Simple Western, IP, ICC/IF |
| RBP1 (RNAPII) (D8L4Y) | 14958 | Cell Signaling | H M R Mk | WB, ChIP |
| RNAPII (4H8) | 101307 | Active Motif | Budding Yeast, C. elegans, Human, Mouse, Rat | WB, IF, ChIP, ICC |
| RPA2 | sc-56770 | Santa Cruz | H M R | WB, IP, IF, IHC(P) and ELISA |
| Vinculin (hVIN-1) | V9131 | Sigma | frog, chicken, mouse, canine, human, bovine, rat, turkey | WB, IF, IHC |
| WRNIP1 (G-2) | 377402 | Santa Cruz | H M R | WB, IP, IF, IHC(P) and ELISA |
| WRNIP1 | A301-389A-T | Bethyl | H | WB, IP, IHC |
| Anti-mouse IgG (Alexa Fluor® 488 Conjugate) | 4408 | Cell Signaling | M | IF |
| Anti-mouse IgG (Alexa Fluor® 555 Conjugate) | 4409 | Cell Signaling | M | IF |
| Anti-rabbit IgG (Alexa Fluor® 488 Conjugate) | 4412 | Cell Signaling | Rabbit | IF |
| Anti-rabbit IgG (Alexa Fluor® 555 Conjugate) | 4413 | Cell Signaling | Rabbit | IF |
| Anti-rat IgG (Alexa Fluor® 488 Conjugate) | 4416 | Cell Signaling | Rat | IF |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | Cell Signaling | Rabbit | WB |
| Anti-mouse IgG, HRP-linked Antibody | 7076 | Cell Signaling | M | WB |
| Anti-rat IgG, HRP-linked Antibody | 7077 | Cell Signaling | Rat | WB |
For western blot, each primary antibody was diluted 1:1000 and secondary antibodies 1:7500. For immunofluorescence and PLA, both primary and secondary antibodies were diluted 1:200.