Table 2.
Oligonucleotides used in this study
| Target | Sequence |
|---|---|
| shWRNIP1 #1 TRCN0000436158 F | CCGGATGATGTGCGAGATGTCATAACTCGAGTTATGACATCTCGCACATCATTTTTTG |
| shWRNIP1 #1 TRCN0000436158 R | AATTCAAAAAATGATGTGCGAGATGTCATAACTCGAGTTATGACATCTCGCACATCAT |
| shWRNIP1 #2 TRCN0000436134 F | CCGGGTGACATTATCTGCAACAAATCTCGAGATTTGTTGCAGATAATGTCACTTTTTG |
| shWRNIP1 #2 TRCN0000436134 R | AATTCAAAAAGTGACATTATCTGCAACAAATCTCGAGATTTGTTGCAGATAATGTCAC |
| shWRNIP1 #3 TRCN0000004527 F | CCGGCGCTGTCGAGTGATTGTTCTTCTCGAGAAGAACAATCACTCGACAGCGTTTTTG |
| shWRNIP1 #3 TRCN0000004527 R | AATTCAAAAACGCTGTCGAGTGATTGTTCTTCTCGAGAAGAACAATCACTCGACAGCG |
| shMYC TRCN0000039642 F | CCGGCCTGAGACAGATCAGCAACAACTCGAGTTGTTGCTGATCTGTCTCAGGTTTTTG |
| shMYC TRCN0000039642 R | AATTCAAAAACCTGAGACAGATCAGCAACAACTCGAGTTGTTGCTGATCTGTCTCAGG |
| shHUWE1 #1F25 | CCGGCCCGCATGATCTTGAATTTCTCGAGAAATTCAAGATCATGCGGGTTTTTG |
| shHUWE1 #1R25 | AATTCAAAAACCCGCATGATCTTGAATTTCTCGAGAAATTCAAGATCATGCGGG |
| shRAD50 #1 TRCN0000040104 F | CCGGCGCCTAAAGAACGACATAGAACTCGAGTTCTATGTCGTTCTTTAGGCGTTTTTG |
| shRAD50 #1 TRCN0000040104 R | AATTCAAAAACGCCTAAAGAACGACATAGAACTCGAGTTCTATGTCGTTCTTTAGGCG |
| shRAD50 #2 TRCN0000009838 F | CCGGGAGATTCGTGATCAGATTACACTCGAGTGTAATCTGATCACGAATCTCTTTTTG |
| shRAD50 #2 TRCN0000009838 R | AATTCAAAAAGAGATTCGTGATCAGATTACACTCGAGTGTAATCTGATCACGAATCTC |
| shScrambled F | CCGGAGGCTCGCATGTATACAGACTCGAGTCTGTATACATGCGAGCCTTTTTTG |
| shScrambled R | AATTCAAAAAAGGCTCGCATGTATACAGACTCGAGTCTGTATACATGCGAGCCT |
| sgRNA HUWE1_130 | AAGGCCCTGCCCAACTCCGT |
| sgRNA HUWE1_230 | CATGCTACTGTTGGCTATCC |
| HA-WRNIP1 cds pRRL F | TGAGTCGGCCGGTGGATCCAATGTACCCTTACGACGTGCCCGACTACGCCGAGGTGAGCGGGCCG |
| HA-WRNIP1 cds pRRL R | GAGGGGCGGATCCGTCGACATCAGCACCTCCTCTGCTTGAAG |
| HA-WRNIP1 trunc1 pRRL F | TGAGTCGGCCGGTGGATCCAATGTACCCTTACGACGTGCCCGACTACGCCCTACAGGGCAAGCCGC |
| HA-WRNIP1 trunc2 pRRL F | TGAGTCGGCCGGTGGATCCAATGTACCCTTACGACGTGCCCGACTACGCTGGGTTGAACGGACTGCAGC |
| HA-WRNIP1 trunc3 pRRL R | GAGGGGCGGATCCGTCGACATCATCGGGCGTCACCGTCACTG |
| HA-WRNIP1 trunc4 pRRL R | GAGGGGCGGATCCGTCGACATCACATCTGTCGGATCTCC |
| HUWE1 clone screen bsr F | GTTGGGATTCGTGAATTGCT |
| HUWE1 clone screen flank R | GGTGTCTTCTTCAGTTTAGTCCTG |
| GAPDH qChIP F | TCCTCTGACTTCAACAGCGAC |
| GAPDH qChIP R | GAGTTGTCAGGGCCCTTTTTC |
| CHEK2 qChIP F | CCACCCTCAGCCAATCAAAAC |
| CHEK2 qChIP R | CCGGGTTCTAAGTTCCGCT |
| PTPN1 qChIP F | GCCCCATGAGCCTTCTGTTA |
| PTPN1 qChIP R | TGCCAAACCGGTCAGAAAGA |
| PARD6B qChIP F | GAGCCCTTCTTCAGGTGCG |
| PARD6B qChIP R | CTCAAAACCCCGCCTACCAC |
| ATP9A qChIP F | CGTGTGAACCACCAGCTATGA |
| ATP9A qChIP R | ATGGCAAGAAAGTTGGCGGG |
| MSL2 qChIP F | CAGGCTTCCCGCATTACACT |
| MSL2 qChIP R | GAGCGGTTCCAGGAGAAAGG |