Fig. 5. Outflow pathway tissue function in autophagy deficient Atg4b^ko mice.

A Toluidine blue-stained histological sections of the iridocorneal region. No morphological abnormalities with Atg4b deficiency were noted. Images are representative of 6 animals per group. TM trabecular meshwork, SC Schlemm’s canal, cb ciliary body. B Mean IOP (mm Hg) monitored over time in Atg4b^wt and Atg4b^ko mice. **p < 0.01, ***p < 0.001, t test (Atg4b^wt n = 20, Atg4b^ko n = 21). C Ex-vivo outflow facility measures in young (4 m.o) and old (18 m.o.) Atg4b^wt and Atg4b^ko mice. *p < 0.05, ANOVA with Bonferroni post hoc test (4 m.o. Atg4b^wt & Atg4b^ko n = 11; 18 m.o. Atg4b^wt & Atg4b^ko n = 9).