Table 6.

ANOVA for Quadratic model.

Source	Sum of squares	df	Mean square	F-value	p-value
Model	17,843.65	14	1274.55	202.91	< 0.0001	Significant
A-pH	1905.49	1	1905.49	303.35	< 0.0001
B-Dose	6255.54	1	6255.54	995.87	< 0.0001
C-Time	81.96	1	81.96	13.05	0.0026
D-Dye	8508.52	1	8508.52	1354.54	< 0.0001
AB	67.28	1	67.28	10.71	0.0051
AC	116.59	1	116.59	18.56	0.0006
AD	25.58	1	25.58	4.07	0.0619
BC	49.04	1	49.04	7.81	0.0136
BD	25.78	1	25.78	4.10	0.0609
CD	0.1502	1	0.1502	0.0239	0.8792
A2	144.14	1	144.14	22.95	0.0002
B2	73.20	1	73.20	11.65	0.0038
C2	45.73	1	45.73	7.28	0.0165
D2	723.21	1	723.21	115.13	< 0.0001
Residual	94.22	15	6.28
Lack of Fit	56.95	10	5.69	0.7639	0.6656	Not significant
Pure error	37.27	5	7.45
Cor total	17,937.88	29