Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Aug 23;11(8):e007082. doi: 10.1136/jitc-2023-007082

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Author(s) (or their employer(s)) 2023. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See http://creativecommons.org/licenses/by-nc/4.0/.

PMC Copyright notice

ISCOMs upregulate distinct pathways in cDC2 and cDC1 subsets. (A) Purity of isolated cDC1s and cDC2s after MACS isolation analyzed by flow cytometry. (B) Top 25 highest expressed genes for cDC1s (left heatmap) and cDC2s (right heatmap) without ISCOMs stimulation based on transformed normalized counts from RNA sequencing. Known genes that are specific for each subset according to literature are depicted in red. (C) Principal component analysis (PCA) for bulk-sequenced cDC1s and cDC2s before and after 8 hours of ISCOMs stimulation. (D) Gene ontology (GO) enrichment for Biological Processes based on DEGs with p<0.05 and fold change ≥2 after stimulation with ISCOMs for 8 hours. Immunologically relevant GO pathways were selected and displayed as a radar plot showing the gene ratio. RNA sequencing for bulk subsets was performed on two to three healthy donors. cDC1, conventional type 1 DC; cDC2, conventional type 2 DC; DEGs, differentially expressed genes; IFN, interferon; IL, interleukin; ISCOMs, immune stimulatory complexes; JAK-STAT, Janus kinases-signal transducer and activator of transcription proteins; MACS, magnetic-activated cell sorting; Th, T helper; VEGF, vascular endothelial growth factor.