Table 2.

Binding affinities of the DBD-HSH tandem domains of TFAP2A (aa 203–420) and TFAP2B (aa 219–457) to different DNA variants

DNA sequences	TFAP2A Kd (nM)	TFAP2B Kd (nM)
5′-GT GCC CGA GGC AG-3′	19 ± 1	18 ± 2
5′-GT cCC CGA GGC AG-3′	78 ± 16	55 ± 7
5′-GT aCC CGA GGC AG-3′	373 ± 46	313 ± 69
5′-GT tCC CGA GGC AG-3′	565 ± 87	248 ± 49
5′-GT cCC CGA GGg AG-3′	336 ± 53	355 ± 57
5′-GT aCC CGA GGt AG-3′	WB	WB
5′-GT tCC CGA GGa AG-3′	WB	WB
5′-GT GaC CGA GGC AG-3′	WB	WB
5′-GT GtC CGA GGC AG-3′	WB	WB
5′-GT GgC CGA GGC AG-3′	WB	WB
5′-GT GCa CGA GGC AG-3′	WB	WB
5′-GT GCg CGA GGC AG-3′	WB	WB
5′-GT GCt CGA GGC AG-3′	694 ± 71	461 ± 71
5′-GT GCt CGA aGC AG-3′	WB	WB
5′-GT atg CGA GGC AG-3′	WB	WB

Only one strand of the DNA duplex is shown in the table, and the mutated nucleotides are shown in lower case. WB: weak binding.