Table 1.

Comparison of the regulation mechanisms of thiM, thiC, and ASW riboswitches between the reported regulation mechanism and our cell-free results from the coupled transcription-translation assays

		Cell-free results
Riboswitch	Mode of regulation	mRNA level	sGFP level
thiM from E. coli	transcriptional &  translational off-switch  (27,28,39)	decreased in presence of TPP	decreased in presence of TPP
thiC from E. coli	transcriptional & translational off-switch (27,29)	decreased in presence of TPP	decreased in presence of TPP
ASW from V. vulnificus	translational on-switch (23–26)	not affected in presence of adenine	increased in presence of adenine