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. 2023 Jul 3;51(15):8115–8132. doi: 10.1093/nar/gkad546

Figure 1.

Figure 1.

CasDinG is a nucleic acid-dependent ATPase. (A) Type IV-A CRISPR operon from P. aeruginosa strain 83 plasmid. (B) V0 plots depicting inorganic phosphate (Pi) production via ATP hydrolysis with 10 nM CasDinG in the presence of 100 nM of different nucleic acid substrates and 600 μM ATP. (C) V0 plot depicting Pi phosphate production via NTP hydrolysis in the presence of 10 nM CasDinG and 600 μM NTP. (D) Michaelis-Menten curve depicting rates of Pi phosphate production at various ATP concentrations in the presence of 100 nM 40 nt ssDNA substrate with 10 nM CasDinG or without any ssDNA (basal). (E) Fluorescence anisotropy curve of CasDinG binding to a 40 nt. 3′- FAM labeled ssDNA with and without AMP-PNP.