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. 2023 Jul 3;51(15):8115–8132. doi: 10.1093/nar/gkad546

Figure 2.

Figure 2.

CasDinG is a 5′-3′ ATP and metal-dependent helicase. (A) Native PAGE helicase assays showing displacement of a FAM-labeled ssDNA or ssRNA from various nucleic acid duplexes. Minimal to no displacement is visualized for 3′ overhangs, blunt-ended duplexes or substrates in which the 5′ overhang is RNA. All reactions were quenched after 20 min. (B) Native PAGE helicase assays showing time course unwinding of 5′-FAM labeled dsDNA 5′-overhang and 5'-FAM labeled RNA/DNA 5'-overhang. The reactions took place for 10 min using 10 nM CasDinG and 15 nM FAM labeled duplexed substrates. (C) A graph plotting the % of displaced FAM-labeled nucleic acids over time with various duplexes. Only the dsDNA 5′-overhang and RNA/DNA hybrid 5′-DNA overhang unwound >50%.