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. 2023 Jul 7;51(15):7714–7735. doi: 10.1093/nar/gkad528

Figure 8.

Figure 8.

The TRIM28, MECP2 and NIPA1 NTARs function in a manner similar to the ERK NTARs in the induction of reporter gene expression. (A) RLU from cell extracts of NIH3T3 cells transfected with constructs harboring the TRIM28 promoter (pTRIM28) without or with its short or long Nt domains fused to ffLuc (sequences are shown beneath the graph with AlaGCG codons in red) (n = 3, representative of three experiments). (B) RLU from cell extracts of NIH3T3 cells transfected with constructs harboring the MECP2 promoter (pMECP2) without or with its Nt domain fused to ffLuc (lanes 1 and 2). In lane 3, alanine codons were mutated to GCG, and in lane 4, alanine codons were mutated to GCA/GCU (sequence of the MECP2 Nt is shown beneath the graph, with AlaGCC codons in green) (n = 3, representative of three experiments). (C) RLU from cell extracts of NIH3T3 cells transfected with constructs harboring the NIPA1 promoter (pNIPA1) without or with its Nt domain fused to ffLuc (lanes 1 and 2). In lane 3, the first two amino acids (G–T) are deleted and in lane 4, two AlaGCG of the stretch are deleted (sequence of the NIPA1 Nt is shown beneath the graph with AlaGCG codons in red) (n = 3, representative of three experiments).