Table 5.
T m values of the duplexes formed between GuNA[R,R]-modified oligonucleotides and the complementary ssRNAa
| Name | Oligonucleotides (5′–3′) | Modification (T = ) | T m (ΔTm/mod.) [°C] | |
|---|---|---|---|---|
| ODN34 | d(GCGTATACGC) | – | 27 | |
| ODN24 | d(GCGTATACGC) | GuNA[Me,Me] | 50 | (+11.5) |
| ODN25 | GuNA[Me,tBu] | 51 | (+12.0) | |
| ODN26 | d(GCGTATACGC) | GuNA[Me,Me] | 63 | (+18.0) |
| ODN27 | GuNA[Me,tBu] | 76 | (+24.5) | |
| ODN28 | d(GTGBrUACAC) | GuNA[Me,Me] | 44 | |
| ODN29 | GuNA[Me,tBu] | 55 | ||
aConditions: 10 mM sodium phosphate buffer (pH 7.2), 100 mM NaCl, 4 μM each oligonucleotide, 0.5°C/min at 260 nm. The Tm values reflect the average of three measurements. ΔTm/mod.: change in Tm value (ΔTm) per modification compared to the unmodified oligonucleotide (ODN34). BrU = 5-bromo-2′-deoxyuridine.