Table 6.
T m Values of the duplexes formed between GuNA[R,R]-modified oligonucleotides and the complementary strandsa
| T m (ΔTm/mod.) [°C] | ||||||
|---|---|---|---|---|---|---|
| Name | Oligonucleotides (5′–3′) | Modification (A or mC = ) | vs. ssRNA | vs. ssDNA | ||
| ODN22 | d(GCGTTATTTGCT) | – | 45 | 49 | ||
| ODN13 | d(GCGTTATTTGCT) | GuNA[Me,Me] | 50 | (+5) | 53 | (+4) |
| ODN14 | GuNA[Me,tBu] | 51 | (+6) | 54 | (+5) | |
| ODN23 | d(GCGTTCTTTGCT) | – | 52 | 53 | ||
| ODN17 | d(GCGTTmCTTTGCT) | GuNA[Me,Me] | 59 | (+7) | 60 | (+7) |
| ODN18 | GuNA[Me,tBu] | 60 | (+8) | 61 | (+8) | |
aConditions: 10 mM sodium phosphate buffer (pH 7.2), 100 mM NaCl, 4 μM each oligonucleotide, 0.5°C/min at 260 nm. Target strands: 5′-r(AGCAAAAAACGC)-3′ and 5′-d(AGCAAAAAACGC)-3′. The Tm values reflect the average of three measurements. ΔTm/mod.: change in Tm value (ΔTm) per modification compared to the unmodified oligonucleotide (ODN22 or ODN23).