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Study of close proximity of Juno and CD9 in mouse oocyte oolemma by PLA. (A) The presence of positive signal (red dots) on the sample stained by Juno and CD9 and visualized by maximal intensity projection, confirmed the existence of their close proximity, (B) α and β tubulin-stained sample was used as a positive control of the method. (C) Combination of α tubulin and CD9 staining was used as a negative control. Chromosomes are visualized with Dapi (blue). Scale bar represents 10 μm.
