Figure 3.

Incorporation of nucleotides opposite 6-oxo-M₁dG by hPolη. Sequence of the DNA duplex with the running start primer (A). Time course of nucleotide incorporation into the running start primer using the control template (B) and the 6-oxo-M₁dG template (C). Increasing concentrations of hPol $\mathrm{\eta }$ (50–200 nM) were incubated with 5 μM DNA duplex and a 500 μM mix of all four dNTPs except for the zero time point, which was incubated with an equal volume of water. The reaction was stopped at 5, 15, and 45 min.