Figure 4.

Steady-state kinetics for dCTP and dGTP incorporation opposite dG or 6-oxo-M₁dG by hPolη. Control (A) and 6-oxo-M₁dG (B) oligonucleotide duplexes (5 μM) were incubated with 10 nM or 200 nM hPol $\mathrm{\eta }$, respectively, and increasing concentrations of dCTP for 6 min for control duplex or 10 min for 6-oxo-M₁dG duplex. C, control (•) and 6-oxo-M₁dG (▲) oligonucleotide duplexes (5 μM) were incubated with 50 nM or 200 nM hPol $\mathrm{\eta }$, respectively, and increasing concentrations of dGTP for 10 min. Each point represents the mean and standard deviation of triplicate determinations. Data were analyzed using the substrate inhibition model in GraphPad Prism.