Figure 5.

Extension of 6-oxo-M₁dG-containing and control oligonucleotide duplexes by hPolη.A, sequence of the duplexes used to evaluate extension past 6-oxo-M₁dG. HPol $\mathrm{\eta }$ was incubated separately or together with a 500 μM mix of all four dNTPs and the indicated control (B) or 6-oxo-M₁dG-containing (C) oligonucleotide duplexes. The polymerase concentrations of hPol $\mathrm{\eta }$ and the oligonucleotide complexes were 200 nM and 5 μM, respectively, and the reactions were stopped after the indicated times. The first three lanes for both the 6-oxo-M₁dG oligonucleotide duplex and control oligonucleotide duplex contained an equal volume of water in place of the added dNTPs.