Table 1.

Steady-state kinetics parameters for insertion of single nucleotides opposite dG and 6-oxo-M₁dG by hPol $\mathrm{\eta }$

Nucleotide	kcat (min−1)	Km (μM)	kcat/Km (μM−1 min−1)	Ki (μM)
dATP	1.9 ± 0.3	47 ± 17	0.040	ND
dCTP	10 ± 2	1.4 ± 0.3	7.1	650 ± 225
dGTP	1.8 ± 0.1	27 ± 7	0.067	6600 ± 2100
dTTP	1.9 ± 0.9	950 ± 540	0.0020	2200 ± 1900
Control oligonucleotide
dATP	0.91 ± 0.12	27 ± 10	0.034	2400 ± 1300
dCTP	0.27 ± 0.05	65 ± 33	0.0042	1800 ± 950
dGTP	0.51 ± 0.03	13 ± 3	0.039	5020 ± 1400
dTTP	1.1 ± 0.3	370 ± 190	0.0030	5000 ± 4200
6-Oxo-M1dG oligonucleotide

Abbreviation: ND, not detectable.